About this course
This 3-day intensive course is tailored for professionals and researchers aiming to master the essential techniques of downstream bioprocessing. We focus on translating theory into tangible skills, making this ideal for anyone seeking to confidently operate, optimize, and troubleshoot key purification and recovery unit operations.
The curriculum is built around direct engagement with industry-standard equipment. You will gain extensive hands-on experience operating a disc-stack centrifuge for cell recovery, a high-pressure homogenizer for cell lysis, and a tangential flow filtration (TFF) system for concentration and diafiltration, alongside essential chromatography methods.
Hosted at the Industrial Biotechnology Innovation Centre (IBioIC), this course combines targeted lectures with immersive practical exercises to build real-world capability. Upon completion of the course, the attendees will be presented with a certificate of course completion by IBioIC.
Learning outcomes
Upon completion of this course, you will be able to:
- Understand the principles and industrial applications of key downstream unit operations, including centrifugation, homogenization, TFF, and chromatography.
- Operate a disc-stack centrifuge for efficient cell harvesting and a high-pressure homogenizer for effective cell lysis.
- Perform concentration and diafiltration (buffer exchange) of a protein solution using a Tangential Flow Filtration (TFF) system.
- Apply chromatography principles to effectively pack a column and execute a purification method using gradient elution.
- Analyse in-process samples to assess process performance and final product purity using protein quantification assays and SDS-PAGE.
- Identify key process parameters for optimization and develop strategies to troubleshoot common downstream processing challenges.
Who will benefit?
- Scientists, engineers, graduates, technical managers, operators, especially those in their early careers in upstream or downstream bioprocessing.
- Anyone who would like to gain an insight into the theoretical and practical aspects of bio-manufacturing.
